Transgenic

Random insertion

利用隨機插入,將特定的片段隨機整合進入genome中,具有容易取得與大量表現特定基因的特性。


HR基因編輯鼠-製作流程

  Process Contents Period
1 Construction of insertion vector 1. Understand customer demand of target genes to design targeting strategies 2. Cloning sequences of insertion vector 3. Linearization of insertion vector for electroporation Approx. 2 months
2 Generation of transgenic mice 4. Pronuclear injection of insertion vector 5. Embryo transfer to pseudopregnant female mouse 6. Generation of transgenic mice 7. Identification of transgenic mice by genotyping Approx. 3 months
3 Delivery 8. Transportation of mice Approx. 1 month

客製化服務

  • Knock out
  • Knock in
  • Over expression
  • Conditional mutant(Cre/Lox base)
  • Inducible system(Tet-On/ off, etc.)
  • Transgene

我們將提供您

  • 每月進度報告
  • 模板載體定序報告
  • 細胞株定序報告
  • 2隻以上基因編輯鼠
  • 基因型鑑定實驗步驟

常見問題

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案例分享

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